Goat Anti-Rabbit IgG (H+L) Antibody (HRP Conjugate)Cat NO.: R00001

Protein names ：Goat Anti-Rabbit IgG (H+L) Antibody (HRP Conjugate)

Form ：Liquid

Purification ：Protein A purification

Host ：Goat

Isotype ：IgG

sensitivity ：Endogenous

Reactivity ：Rabbit

• ApplicationDilution
• 免疫印迹(WB)1：5000
• The optimal dilutions should be determined by the end user

Specificity ：The antibody was developed in goat using the Rabbit IgG as the immunogen.

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

Function ：Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found i

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.