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Western blot(SDS PAGE) analysis of extracts from (1) JAR cell treated with Lambda Phosphatase lysate; (2) JAR cell lysate.Using P-Cyclin E1 (T77) Rabbit mAb [7EO8]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CCNE; Ccne1; cyclin E variant ex5del; cyclin E variant ex7del; Cyclin E1; Cyclin Es; Cyclin Et; G1/S specific cyclin E; G1/S-specific cyclin-E1;
UniProtID :P24864
MASS(da) :47,077
MW(kDa) :48kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Cyclin E1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) JAR cell treated with Lambda Phosphatase lysate; (2) JAR cell lysate.
Function : Essential for the control of the cell cycle at the G1/S (start) transition..
Tissue specificity :Highly expressed in testis and placenta. Low levels in bronchial epithelial cells..
Subcellular locationi :Nucleus.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.