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Western blot(SDS PAGE) analysis of extracts from (1) Jurkat cell lysate treated with Alkaline Phosphatase; (2) Jurkat cell lysate.Using P-CDC37 (S13) Rabbit mAb [6PQg]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CC37; Hsp90 chaperone protein kinase-targeting subunit; Hsp90 co-chaperone Cdc37;
UniProtID :Q16543
MASS(da) :44,468
MW(kDa) :50kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Phospho-CDC37 (S13)
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) Jurkat cell lysate treated with Alkaline Phosphatase; (2) Jurkat cell lysate.
Function : Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity (PubMed:8666233). Inhibits HSP90AA1 ATPase activity (PubMed:23569206)..
Subcellular locationi :Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.