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Western blot(SDS PAGE) analysis of extracts from (1) SH-SY5Y cell lysate; (2) Mouse kidney lysate.Using DRD1 Rabbit mAb [tYcz]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :D(1A) dopamine receptor; DADR; Dopamine D1 receptor; DR D1Al; DRD1; DRD1A;
UniProtID :P21728
MASS(da) :49,293
MW(kDa) :75kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human DRD1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) SH-SY5Y cell lysate; (2) Mouse kidney lysate.
Function : Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
Tissue specificity :Detected in caudate, nucleus accumbens and in the olfactory tubercle..
Subcellular locationi :Cell membrane,Multi-pass membrane protein. Endoplasmic reticulum membrane,Multi-pass membrane protein. Cell projection, dendrite. Cell projection, dendritic spine.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.