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Western blot(SDS PAGE) analysis of extracts from HepG2 cell lysate.Using GCLC Rabbit mAb [0NBH]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GCS heavy chain; Gamma-ECS Gamma-glutamylcysteine synthetase; GLCL; GLCLC; GCLC
UniProtID :P48506
MASS(da) :72,766
MW(kDa) :73kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human GCLC
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cell lysate.
Function : Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.