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Western blot(SDS PAGE) analysis of extracts from MCF7 cell lysate.Using GCET1 Rabbit mAb [Zu7c]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Centerin; GCET1; Seprin A9; SERPINA11; SERPINA11b; Serpina9;
UniProtID :Q86WD7
MASS(da) :46,557
MW(kDa) :47kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human GCET1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF7 cell lysate.
Function : Protease inhibitor that inhibits trypsin and trypsin-like serine proteases (in vitro). Inhibits plasmin and thrombin with lower efficiency (in vitro)..
Tissue specificity :Highly expressed in normal germinal center (GC) B-cells and GC B-cell-derived malignancies..
Subcellular locationi :[Isoform 1]: Secreted.,[Isoform 2]: Cytoplasm.,[Isoform 3]: Cytoplasm.,[Isoform 4]: Cytoplasm.,[Isoform 5]: Cytoplasm.,[Isoform 6]: Cytoplasm.,[Isoform 7]: Membrane,Single-pass type II membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.