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Western blot(SDS PAGE) analysis of extracts from TNF beta recombinant protein.Using TNF beta Rabbit mAb [2yNX]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :LTalpha; DIF; LT alpha; Lta; Lymphotoxin alpha; TNF B; TNF superfamily member 1; TNF-beta; TNFB; TNFbeta; TNFSF1; TNLG1E;
UniProtID :P01374
MASS(da) :22,297
MW(kDa) :18kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human TNF beta
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :TNF beta recombinant protein.
Function : Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM (PubMed:9462508). In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and is cytotoxic for a wide range of tumor cells in vitro and in vivo..
Subcellular locationi :Secreted. Membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.