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Western blot(SDS PAGE) analysis of extracts from (1) 293T cell lysate; (2) RAW 264 .7 cell lysate.Using DOPA Decarboxylase Rabbit mAb [l0X9]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :AADC; Aromatic L Amino Acid Decarboxylase; DDC; DOPA decarboxylase (aromatic L-amino acid decarboxylase); DOPA decarboxylase;
UniProtID :P20711
MASS(da) :53,926
MW(kDa) :53kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human DOPA Decarboxylase
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) 293T cell lysate; (2) RAW 264 .7 cell lysate.
Function : Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine and L-5-hydroxytryptophan to serotonin..
Tissue specificity :[Isoform 2]: High expression in kidney..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.