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Western blot(SDS PAGE) analysis of extracts from HepG2 cell lysate.Using Protein C Rabbit mAb [0KLJ]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Activation peptide; Anticoagulant protein C; APC; Autoprothrombin IIA; Blood coagulation factor XIV; PROC1; THPH3; THPH4; Vitamin K dependent protein C;
UniProtID :P04070
MASS(da) :52,071
MW(kDa) :52kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Protein C
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cell lysate.
Function : Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845)..
Tissue specificity :Plasma,synthesized in the liver.
Subcellular locationi :Secreted. Golgi apparatus. Endoplasmic reticulum.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.