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Western blot(SDS PAGE) analysis of extracts from A375 cell lysate.Using BCHE Rabbit mAb [DE33]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :BCHE; Butyrylcholine esterase; CHE1; CHE2; Choline esterase II; Cholinesterase (serum) 2; Cholinesterase 1; Cholinesterase; E1;
UniProtID :P06276
MASS(da) :68,418
MW(kDa) :90kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human BCHE
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A375 cell lysate.
Function : Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters..
Tissue specificity :Detected in blood plasma (at protein level). Present in most cells except erythrocytes..
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.