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Western blot(SDS PAGE) analysis of extracts from HepG2 cell lysate.Using Follistatin Rabbit mAb [1n42]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Activin binding protein; Follistatin; FS; fst; Human follistatin gene;
UniProtID :P19883
MASS(da) :38,007
MW(kDa) :38kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Follistatin
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cell lysate.
Function : Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).
Tissue specificity :Isoform 1 is the predominant isoform in serum but is undetectable in follicular fluid. In the embryo, strong expression is seen in the palatal epithelia, including the medial edge epithelial and midline epithelial seam of the palatal shelves. Less pronounced expression is also seen throughout the palatal shelf and tongue mesenchyme (PubMed:31215115)..
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.