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Western blot(SDS PAGE) analysis of extracts from human fetal brain lysate.Using Muscarinic Acetylcholine Receptor M3 Rabbit mAb [Af1h]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :AChR; CHRM3; EGBRS; HM3; M3 muscarinic receptor;
UniProtID :P20309
MASS(da) :66,128
MW(kDa) :66kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Muscarinic Acetylcholine Receptor M3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :human fetal brain lysate.
Function : The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover..
Subcellular locationi :Cell membrane,Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane,Multi-pass membrane protein. Basolateral cell membrane,Multi-pass membrane protein. Endoplasmic reticulum membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.