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Western blot(SDS PAGE) analysis of extracts from (1)A431 cell lysate; (2)NIH/3T3 cell lysate.Using TPOR Rabbit mAb [ZvvP]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :C MPL; CD110; mpl; MPLV; THCYT2; Thrombopoietin receptor; TPO R; TPOR;
UniProtID :P40238
MASS(da) :71,245
MW(kDa) :82kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human TPOR
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1)A431 cell lysate; (2)NIH/3T3 cell lysate.
Function : Receptor for thrombopoietin that acts as a primary regulator of megakaryopoiesis and platelet production. May represent a regulatory molecule specific for TPO-R-dependent immune responses..
Tissue specificity :Expressed at a low level in a large number of cells of hematopoietic origin. Isoform 1 and isoform 2 are always found to be coexpressed.
Subcellular locationi :Cell membrane,Single-pass type I membrane protein. Golgi apparatus. Cell surface.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.