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Western blot(SDS PAGE) analysis of extracts from Rat muscle cell lysate.Using FNDC5 Rabbit mAb [7uH5]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :FNDC 5; FRCP2; irisin;
UniProtID :Q8NAU1
MASS(da) :23,659
MW(kDa) :24kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human FNDC5
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Rat muscle cell lysate.
Function : [Irisin]: Contrary to mouse, may not be involved in the beneficial effects of muscular exercise, nor in the induction of browning of human white adipose tissue..
Tissue specificity :Widely expressed, with highest levels in heart. Very low expression, if any, in colon, pancreas and spleen..
Subcellular locationi :Cell membrane,Single-pass type I membrane protein. Peroxisome membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.