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EBF1 Rabbit mAb [Zw6y]Cat NO.: A33696

Western blot(SDS PAGE) analysis of extracts from Raji cell lysate.Using EBF1 Rabbit mAb [Zw6y]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :COE1; EBF; Ebf1; O/E 1; OE1; OLF1;

UniProtID :Q9UH73

MASS(da) :64,464

MW(kDa) :64kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human Mouse

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human EBF1

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :Raji cell lysate.

Function : Key pioneer transcription factor of B-cell specification and commitment (PubMed:27807034). Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Operates in a transcription factor network to activate B-cell-specific genes and repress genes associated with alternative cell fates. For instance, positively regulates many B-cell specific genes including BCR or CD40 while repressing genes that direct cells into alternative lineages, including GATA3 and TCF7 for the T-cell lineage. In addition to its role during lymphopoiesis, controls the thermogenic gene program in adipocytes during development and in response to environmental cold (By similarity).., (Microbial infection) Acts as a chromatin anchor for Epstein-Barr virus EBNA2 to mediate the assembly of EBNA2 chromatin complexes in B-cells (PubMed:28968461). In addition, binds to the viral LMP1 proximal promoter and promotes its expression during latency (PubMed:26819314)..

Subcellular locationi :Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.