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Western blot(SDS PAGE) analysis of extracts from mouse skeletal muscle lysate.Using ACTN3 Rabbit mAb [E4Z2]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Actinin alpha 3; ACTN3;
UniProtID :Q08043
MASS(da) :103,241
MW(kDa) :103 kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human ACTN3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :mouse skeletal muscle lysate.
Function : F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Tissue specificity :Expressed only in a subset of type 2 skeletal muscle fibers..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.