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UNG Rabbit mAb [38F5]Cat NO.: A27142

Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using UNG Rabbit mAb [38F5]at dilution of 1:1000 incubated at 4℃ over night.

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Product information

Protein names :UNG; DGU; HIGM4; HIGM5; Uracil-DNA glycosylase; UNG1; UNG2; UDG; UNG15;

UniProtID :P13051

MASS(da) :34,645

MW(kDa) :32kDa

Form :Liquid

Purification :Affinity-chromatography

Host :Rabbit

Isotype : IgG

sensitivity :Endogenous

Reactivity :Human,Mouse,Rat

  • ApplicationDilution
  • 免疫印迹(WB)1:1000-2000
  • 免疫组化(IHC)1:100
  • 免疫荧光(ICC/IF)1:100
  • The optimal dilutions should be determined by the end user

Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human UNG

Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected :HeLa cell lysate.

Function : Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.

Tissue specificity :Isoform 1 is widely expressed with the highest expression in skeletal muscle, heart and testicles. Isoform 2 has the highest expression levels in tissues containing proliferating cells.

Subcellular locationi :[Isoform 1]: Mitochondrion.,[Isoform 2]: Nucleus.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.