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Western blot(SDS PAGE) analysis of extracts from Ramos cell lysate.Using PLCG2 Rabbit mAb [91Ud]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :PLC 2; PLC gamma 2; PLC IV; PLC-IV; Plcg2;
UniProtID :P16885
MASS(da) :147,870
MW(kDa) :148kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human PLCG2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Ramos cell lysate.
Function : The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.