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Western blot(SDS PAGE) analysis of extracts from SH-SY5Y cell lysate.Using DCTN3 Rabbit mAb [QskK]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :DCNT22; DCTN22; Dctn3; dynactin 3 (p22); dynactin light chain; Dynactin subunit 3; p22;
UniProtID :O75935
MASS(da) :21,119
MW(kDa) :21kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human DCTN3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SH-SY5Y cell lysate.
Function : Together with dynein may be involved in spindle assembly and cytokinesis..
Tissue specificity :Ubiquitously expressed. Highly expressed in muscle and pancreas and detected at lower levels in brain..
Subcellular locationi :Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Cleavage furrow. Midbody.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.