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Western blot(SDS PAGE) analysis of extracts from A431 cell lysate.Using Desmoglein 3 Rabbit mAb [Jg23]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CDHF6; Desmoglein3; DSG3; PVA;
UniProtID :P32926
MASS(da) :107,533
MW(kDa) :145kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Desmoglein 3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A431 cell lysate.
Function : Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Tissue specificity :Epidermis, tongue, tonsil, esophagus and carcinomas. Expressed in skin and mucosa (at protein level) (PubMed:30528827)..
Subcellular locationi :Cell membrane,Single-pass type I membrane protein. Cell junction, desmosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.