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Western blot(SDS PAGE) analysis of extracts from (1) MCF7 cell lysate; (2) RAW264.7 cell lysate.Using DNA Polymerase gamma Rabbit mAb [Ppf8]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MDP1; PEO; PolG alpha; POLG; POLG1; POLGA; SANDO; SCAE;
UniProtID :P54098
MASS(da) :139,562
MW(kDa) :140kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human DNA Polymerase gamma
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) MCF7 cell lysate; (2) RAW264.7 cell lysate.
Function : Involved in the replication of mitochondrial DNA. Associates with mitochondrial DNA.
Subcellular locationi :Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.