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Western blot(SDS PAGE) analysis of extracts from U-87MG cell lysate.Using 5HT7 Receptor Rabbit mAb [zIBz]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :5 HT 7; 5 HT X; 5-HT-7; 5-HT-X; 5-HT7; 5HT7; htr7;
UniProtID :P34969
MASS(da) :53,555
MW(kDa) :54kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human 5HT7 Receptor
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :U-87MG cell lysate.
Function : This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.
Tissue specificity :Isoform A is the predominant isoform in spleen, caudate and hippocampus. Isoform B is expressed at lower levels. Isoform D is a minor isoform in terms of expression..
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.