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Western blot(SDS PAGE) analysis of extracts from Hela cell lysate.Using RhoGAP Rabbit mAb [718T]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ARHGAP5; p190 B; p190 RhoGAP; Rho GTPase activating protein 5; RhoGAP; RhoGAP5;
UniProtID :Q13017
MASS(da) :172,460
MW(kDa) :190kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human RhoGAP
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Hela cell lysate.
Function : GTPase-activating protein for Rho family members (PubMed:8537347)..
Tissue specificity :Detected in skin fibroblasts (at protein level) (PubMed:8537347)..
Subcellular locationi :Cytoplasm. Cell membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.