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Western blot(SDS PAGE) analysis of extracts from (1) Jurkat cell lysate; (2) Molt-4 cell lysate.Using PI 3 Kinase R4 Rabbit mAb [6tiX]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :P150; Phosphoinositide 3 kinase regulatory subunit 4; PI3 kinase p150 subunit; PIK3R4; VPS15;
UniProtID :Q99570
MASS(da) :153,103
MW(kDa) :150kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human PI 3 Kinase R4
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) Jurkat cell lysate; (2) Molt-4 cell lysate.
Function : Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate,different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123)..
Tissue specificity :Ubiquitously expressed..
Subcellular locationi :Late endosome. Cytoplasmic vesicle, autophagosome. Membrane,Lipid-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.