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Western blot(SDS PAGE) analysis of extracts from Hela cell lysate.Using ARF5 Rabbit mAb [39Zj]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :ADP ribosylation factor 5; Arf5;
UniProtID :P84085
MASS(da) :20,530
MW(kDa) :18kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human ARF5
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Hela cell lysate.
Function : GTP-binding protein involved in protein trafficking,may modulate vesicle budding and uncoating within the Golgi apparatus.., (Microbial infection) Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase..
Subcellular locationi :Golgi apparatus. Cytoplasm, perinuclear region. Membrane,Lipid-anchor. Golgi apparatus, trans-Golgi network membrane,Lipid-anchor.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.