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Western blot(SDS PAGE) analysis of extracts from Jurkat cell lysate.Using VPS28 Rabbit mAb [yHii]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :H-Vps28; Vps28;
UniProtID :Q9UK41
MASS(da) :25,425
MW(kDa) :25kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human VPS28
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Jurkat cell lysate.
Function : Component of the ESCRT-I complex, a regulator of vesicular trafficking process..
Subcellular locationi :Cell membrane. Late endosome membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.