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Western blot(SDS PAGE) analysis of extracts from HepG2 cell lysate.Using Glycerol kinase Rabbit mAb [0pdK]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GK1; GKD; Glycerokinase;
UniProtID :P32189
MASS(da) :61,245
MW(kDa) :50kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Glycerol kinase
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cell lysate.
Function : Key enzyme in the regulation of glycerol uptake and metabolism.
Tissue specificity :Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
Subcellular locationi :Mitochondrion outer membrane,Peripheral membrane protein,Cytoplasmic side. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.