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Western blot(SDS PAGE) analysis of extracts from Human fetal heart lysate.Using Nidogen 1 Rabbit mAb [2zx3]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Enactin; Ent; Entactin 1; Entactin; NID 1; NID; Nid1; Nidogen 1; Nidogen;
UniProtID :P14543
MASS(da) :136,377
MW(kDa) :160kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Nidogen 1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Human fetal heart lysate.
Function : Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
Subcellular locationi :Secreted, extracellular space, extracellular matrix, basement membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.