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Western blot(SDS PAGE) analysis of extracts from MCF-7 cell lysate.Using GJB1 Rabbit mAb [2GW8]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CMTX; CMTX1; Connexin32; Cx32; GJB1;
UniProtID :P08034
MASS(da) :32,025
MW(kDa) :32kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human GJB1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :MCF-7 cell lysate.
Function : One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
Subcellular locationi :Cell membrane,Multi-pass membrane protein. Cell junction, gap junction.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.