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Western blot(SDS PAGE) analysis of extracts from K562 cell lysate.Using COL11A1 Rabbit mAb [Zd6e]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :COL11A1; COLL6; Collagen alpha 1; collagen XI alpha 1; STL2; STL3;
UniProtID :P12107
MASS(da) :181,065
MW(kDa) :150kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human COL11A1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K562 cell lysate.
Function : May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.
Tissue specificity :Cartilage, placenta and some tumor or virally transformed cell lines. Isoforms using exon IIA or IIB are found in the cartilage while isoforms using only exon IIB are found in the tendon.
Subcellular locationi :Secreted, extracellular space, extracellular matrix.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.