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Western blot(SDS PAGE) analysis of extracts from mouse eyeball lysate.Using Cytokeratin 12 Rabbit mAb [UiPw]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :K12; Keratin 12; KRT12; type I cytoskeletal 12;
UniProtID :Q99456
MASS(da) :53,511
MW(kDa) :54kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Cytokeratin 12
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :mouse eyeball lysate.
Function : Involved in corneal epithelium organization, integrity and corneal keratin expression..
Tissue specificity :Expressed in the corneal epithelium (at protein level)..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.