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Western blot(SDS PAGE) analysis of extracts from A431 cell lysate.Using Desmocollin 3 Rabbit mAb [wgEJ]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CDHF3; Desmocollin3; Desmocollin4; DSC; DSC1; DSC2; DSC3; DSC4; HTCP;
UniProtID :Q14574
MASS(da) :99,969
MW(kDa) :100kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from Desmocollin 3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A431 cell lysate.
Function : Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms.
Tissue specificity :Epidermis, buccal mucosa, esophagus and cervix.
Subcellular locationi :Cell membrane,Single-pass type I membrane protein. Cell junction, desmosome.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.