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Western blot(SDS PAGE) analysis of extracts from (1) HeLa cell lysate; (2) 293 cell lysate.Using UPP1 Rabbit mAb [236Z]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :UDRPASE; UPase 1; UPASE; UPP; Upp1; UrdPase 1;
UniProtID :Q16831
MASS(da) :33,934
MW(kDa) :28kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human UPP1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) HeLa cell lysate; (2) 293 cell lysate.
Function : Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate (PubMed:7488099). The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis..
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.