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Western blot(SDS PAGE) analysis of extracts from HeLa cell lysate.Using HFE Rabbit mAb [TH9I]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Hemochromatosis; HFE; HFE1; HLAH; MVCD7; TFQTL2;
UniProtID :Q30201
MASS(da) :40,108
MW(kDa) :40kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human HFE
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cell lysate.
Function : Binds to transferrin receptor (TFR) and reduces its affinity for iron-loaded transferrin..
Tissue specificity :Expressed in all tissues tested except brain.
Subcellular locationi :Cell membrane,Single-pass type I membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.