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Western blot(SDS PAGE) analysis of extracts from Ramos cell lysate.Using NPY5R Rabbit mAb [VH35]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :NPY 5R; NPY R5; NPY Y5; NPY Y5 receptor; NPY5R; NPYR5; NPYY5;Y5 receptor;
UniProtID :Q15761
MASS(da) :50,727
MW(kDa) :51kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human NPY5R
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :Ramos cell lysate.
Function : Receptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders.
Tissue specificity :Brain,hypothalamus.
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.