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Western blot(SDS PAGE) analysis of extracts from (1) HeLa cell lysate; (2) Mouse brain lysate.Using SNAP29 Rabbit mAb [iPqY]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CEDNIK; SNAP29;
UniProtID :O95721
MASS(da) :28,970
MW(kDa) :27kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human SNAP29
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) HeLa cell lysate; (2) Mouse brain lysate.
Function : SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. SNAP29 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Plays also a role in ciliogenesis by regulating membrane fusions..
Tissue specificity :Found in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas..
Subcellular locationi :Cytoplasm. Golgi apparatus membrane,Peripheral membrane protein. Cytoplasmic vesicle, autophagosome membrane,Peripheral membrane protein. Cell projection, cilium membrane,Peripheral membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.