HIF Prolyl Hydroxylases Rabbit mAb [63V8]Cat NO.: A54640

Protein names ：EGLN4 ; HIFPH4; Hypoxia inducible factor prolyl 4 hydroxylase;P4H with transmembrane domain; P4htm; PH4; PHD4; Proline 4 hydroxylase; Prolyl hydroxlase domain containing 4;

UniProtID ：Q9NXG6

MASS(da) ：56,661

MW(kDa) ：47kDa

Form ：Liquid

Purification ：Affinity-chromatography

Host ：Rabbit

Isotype ： IgG

sensitivity ：Endogenous

Reactivity ：Human

• ApplicationDilution
• 免疫印迹(WB)1:1000-2000
• 免疫组化(IHC)1:100
• The optimal dilutions should be determined by the end user

Specificity ：Antibody is produced by immunizing animals with A synthesized peptide derived from human HIF Prolyl Hydroxylases

Storage ：Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.

WB Positive detected ：HeLa cell lysate.

Function ： Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex..

Tissue specificity ：Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts..

Subcellular locationi ：Endoplasmic reticulum membrane,Single-pass type II membrane protein.

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.