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Western blot(SDS PAGE) analysis of extracts from HEK293 cell lysate.Using NDUFB9 Rabbit mAb [5K1R]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :complex I B22 subunit;LYR motif containing protein 3; LYRM3; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9; NADH ubiquinone oxidoreductase B22 subunit; Ndufb9; UQOR22;
UniProtID :Q9Y6M9
MASS(da) :21,831
MW(kDa) :22kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human NDUFB9
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HEK293 cell lysate.
Function : Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone..
Subcellular locationi :Mitochondrion inner membrane,Peripheral membrane protein,Matrix side.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.