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Western blot(SDS PAGE) analysis of extracts from HepG2 cell lysate.Using ENDOGL1 / ENGL Rabbit mAb [JjEK]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Endo G like 1; ENDOGL1; ENDOGL2; Endonuclease G like 1; Endonuclease G like 2; ENGL;ENGLA; ENGLB; Exog;Nuclease EXOG;
UniProtID :Q9Y2C4
MASS(da) :41,085
MW(kDa) :41kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human ENDOGL1 / ENGL
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cell lysate.
Function : Endo/exonuclease with nicking activity towards supercoiled DNA, a preference for single-stranded DNA and 5'-3' exonuclease activity..
Tissue specificity :Ubiquitous.
Subcellular locationi :Mitochondrion inner membrane.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.