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Western blot(SDS PAGE) analysis of extracts from HepG2 cell lysate.Using PLCB1 Rabbit mAb [q98g]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase beta 1; 1-phosphatidylinositol 4; EIEE12; Inositoltrisphosphohydrolase; Monophosphatidylinositol phosphodiesterase; Phosphb; Phosphoinositidase C ; Phosphoinositide phospholipase C; Phosphoinositide phospholipase C-beta 1; Phospholipase C beta 1 (phosphoinositide-specific); Phospholipase C I; Phospholipase C-beta-1; Phospholipase C-I; PI PLC; PLC 1; PLC beta 1;PLC-154; PLC-beta-1; PLC-I; PLC154; Plcb; Plcb1;PLCbeta1; Triphosphoinositide phosphodiesterase;
UniProtID :Q9NQ66
MASS(da) :138,567
MW(kDa) :100,150kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human PLCB1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HepG2 cell lysate.
Function : Catalyzes the hydrolysis of 1-phosphatidylinositol 4,5-bisphosphate into diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) and mediates intracellular signaling downstream of G protein-coupled receptors (PubMed:9188725). Regulates the function of the endothelial barrier..
Subcellular locationi :Nucleus membrane. Cytoplasm.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.