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Western blot(SDS PAGE) analysis of extracts from A549 cell lysate.Using OXGR1 / GPR99 Rabbit mAb [tPmq]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :GPR80; GPR99; Oxgr1; P2RY15; P2Y15;
UniProtID :Q96P68
MASS(da) :38,251
MW(kDa) :36kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human OXGR1 / GPR99
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :A549 cell lysate.
Function : Receptor for alpha-ketoglutarate. Seems to act exclusively through a G(q)-mediated pathway (By similarity)..
Tissue specificity :Detected in kidney and, to a lower extent, in placenta. Not detected in brain tissues including the frontal cortex, caudate putamen, thalamus, hypothalamus, hippocampus or pons.
Subcellular locationi :Cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.