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Western blot(SDS PAGE) analysis of extracts from 293 cells.Using CISD1/mitoNEET Rabbit mAb [IV13] at dilution of 1:1000 incubated at 4℃ over night.
Protein names :CISD1,C10orf70,ZCD1,MDS029,CISD1_HUMAN,CDGSH iron-sulfur domain-containing protein 1
UniProtID :Q9NZ45
MASS(da) :12,199
MW(kDa) :12 kDa
Form :Liquid
Purification :Protein A purification
Host :Rabbit
Isotype :IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with a synthetic peptide at the sequence of Human CISD1/mitoNEET
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :293 cells
Function : Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity). May be involved in Fe-S cluster shuttling and/or in redox reactions..
Tissue specificity :Expression is reduced in cells derived from cystic fibrosis patients..
Subcellular locationi :Mitochondrion outer membrane,Single-pass type III membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.