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Western blot(SDS PAGE) analysis of extracts from K562 cell lysate.Using SLC12A1 / NKCC2 Rabbit mAb [43s1]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :BSC1; NKCC2; Slc12a1;
UniProtID :Q13621
MASS(da) :121,450
MW(kDa) :150kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human SLC12A1 / NKCC2
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :K562 cell lysate.
Function : Renal sodium, potassium and chloride ion cotransporter that mediates the transepithelial NaCl reabsorption in the thick ascending limb and plays an essential role in the urinary concentration and volume regulation. Electrically silent transporter system..
Tissue specificity :Kidney,localizes to the thick ascending limbs (at protein level)..
Subcellular locationi :Apical cell membrane,Multi-pass membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.