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Western blot(SDS PAGE) analysis of extracts from (1) Raji cell lysate; (2) RAW 264.7 cell lysate; (3) C6 cell lysate.Using PRPF4 Rabbit mAb [U8Wk]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :hPrp4; HPRP4P; PRP4; Prp4p; PRPF4; RP70; SNRNP60;
UniProtID :O43172
MASS(da) :58,449
MW(kDa) :58kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human PRPF4
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) Raji cell lysate; (2) RAW 264.7 cell lysate; (3) C6 cell lysate.
Function : Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex)..
Subcellular locationi :Nucleus. Nucleus speckle.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.