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Western blot(SDS PAGE) analysis of extracts from (1) 293 cell lysate; (2) Mouse brain lysate.Using CSDE1 Rabbit mAb [JLZ9]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Csde1; NRU; UNR;
UniProtID :O75534
MASS(da) :88,885
MW(kDa) :89kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human CSDE1
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :(1) 293 cell lysate; (2) Mouse brain lysate.
Function : RNA-binding protein involved in translationally coupled mRNA turnover (PubMed:11051545, PubMed:15314026). Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (PubMed:11051545, PubMed:15314026). Required for efficient formation of stress granules (PubMed:29395067).., (Microbial infection) Required for internal initiation of translation of human rhinovirus RNA..
Subcellular locationi :Cytoplasm. Cytoplasm, Stress granule. Cytoplasm, P-body.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.