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Western blot(SDS PAGE) analysis of extracts from SH-SY5Y cell lysate.Using Dopamine beta Hydroxylase Rabbit mAb [7j6b]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :dbh; DBM; Dopamine beta hydroxylase; Dopamine beta monooxygenase;Soluble dopamine beta-hydroxylase;
UniProtID :P09172
MASS(da) :69,065
MW(kDa) :70-75kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Dopamine beta Hydroxylase
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :SH-SY5Y cell lysate.
Function : Conversion of dopamine to noradrenaline..
Subcellular locationi :[Soluble dopamine beta-hydroxylase]: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. Secreted.,Cytoplasmic vesicle, secretory vesicle membrane,Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane,Single-pass type II membrane protein.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.