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Western blot(SDS PAGE) analysis of extracts from recombinant protein cell lysate.Using CCL23 / MIP3 Rabbit mAb [F21I]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :C C motif chemokine ligand 23; C-C motif chemokine 23; C6 beta chemokine; CCL23; CCL23(30-99); Chemokine (C C motif) ligand 23; Chemokine CC motif ligand 23; ck beta 8;CKB8; Hmrp-2a; Macrophage inflammatory protein 3; MIP3; MPIF1; Myeloid progenitor inhibitory factor 1;SCYA23; Small inducible cytokine A 23; Small inducible cytokine subfamily A, member 23; Small-inducible cytokine A23;
UniProtID :P55773
MASS(da) :13,443
MW(kDa) :13kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human CCL23 / MIP3
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :recombinant protein cell lysate.
Function : Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23..
Tissue specificity :High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are found in high levels in synovial fluids from rheumatoid patients..
Subcellular locationi :Secreted.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.