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Western blot(SDS PAGE) analysis of extracts from various lysates.Using P-MAP2 (S136) Rabbit mAb [9Y7z]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :MAP 2; MAP dendrite specific;MAP2; MAP2A; MAP2B; MAP2C; Microtubule associated protein 2; Microtubule-associated protein 2;
UniProtID :P11137
MASS(da) :199,526
MW(kDa) :70/280kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Phospho-MAP2 (S136)
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :various lysates
Function : The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Subcellular locationi :Cytoplasm, cytoskeleton. Cell projection, dendrite.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.