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Western blot(SDS PAGE) analysis of extracts from HeLa cells, .Using P-PKA C (Thr197) Rabbit mAb [qqfB]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :cAMP dependent protein kinase catalytic subunit alpha; cAMP dependent protein kinase catalytic subunit beta; cAMP dependent protein kinase catalytic subunit gamma; PKA C alpha; PKA C beta; PKA C gamma; PKACA; PRKACA; PRKACB; PRKACG;
UniProtID :P22612/P17612/P22694
MASS(da) :40,434
MW(kDa) :40kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human Phospho-PKA C (Thr197)
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :HeLa cells,
Function : Phosphorylates a large number of substrates in the cytoplasm and the nucleus.
Tissue specificity :Testis specific. But important tissues such as brain and ovary have not been analyzed for the content of transcript.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.