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Western blot(SDS PAGE) analysis of extracts from various lysates.Using SNAP25 Rabbit mAb [91xq]at dilution of 1:1000 incubated at 4℃ over night.
Protein names :Bdr; Resistance to inhibitors of cholinesterase 4 homolog; RIC4; SEC9; SNAP 25; SNAP;SNAP25; SNP25;SUP; Super protein; Synaptosomal associated 25 kDa protein; Synaptosomal associated protein; Synaptosomal associated protein 25kDa; Synaptosomal-associated 25 kDa protein; Synaptosomal-associated protein 25; Synaptosomal-associated protein;
UniProtID :P60880
MASS(da) :23,315
MW(kDa) :25kDa
Form :Liquid
Purification :Affinity-chromatography
Host :Rabbit
Isotype : IgG
sensitivity :Endogenous
Reactivity :Human,Mouse,Rat
Specificity :Antibody is produced by immunizing animals with A synthesized peptide derived from human SNAP25
Storage :Antibody store in 10 mM PBS, 0.5mg/ml BSA, 50% glycerol. Shipped at 4°C. Store at-20°C or -80°C. Products are valid for one natural year of receipt.Avoid repeated freeze / thaw cycles.
WB Positive detected :various lysates
Function : t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells..
Tissue specificity :Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
Subcellular locationi :Cytoplasm, perinuclear region. Cell membrane,Lipid-anchor. Cell junction, synapse, synaptosome. Photoreceptor inner segment.
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 1% w/v BSA, 1X TBST at 4°C overnight.